ITC binding isotherms from titrations of FMN, RF and FAD with Apo-YLOV.

<p>Titrations of 400 µM Apo-YLOV into 40 µM FMN (A), 40 µM RF (B) and 40 µM FAD (C) were performed twice and heats of reaction are represented in the upper diagrams by black and red dots, respectively. All titration curves were fitted for a single binding site.The individual binding isotherms (black and red lines) were obtained from a global fit of the combined data from experiments belonging to titrations with the same flavin. The corresponding residuals (rmsd) are displayed at the bottom. All calculated values are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081268#pone-0081268-t002" target="_blank">Table 2</a>.