Free cholesterol induction of ER stress in adipocytes.

<p>Fully differentiated 3T3-L1 cells were incubated in the medium containing various concentration of ox-LDL (0-100µg/ml) with Sandoz 58035 for 48 hrs (A). Lane 1: control; lane 2: ox-LDL at 25μg/ml; lane 3: ox-LDL at 50μg/ml; lane 4: ox-LDL at 75μg/ml; lane 5: ox-LDL at 100μg/ml; Adipocytes were incubated with ox-LDL (50µg/ml) in a combination of Sandoz58035 for indicated times (0-48hrs) (B). Lane 1: control; lane 2: ox-LDL for 6h; lane 3: ox-LDL for 18h; lane 4: ox-LDL for 36h; lane 5: ox-LDL for 48h. GRP78 and CHOP expression was assessed by Western Blot andβ-actin was used as the housekeeping gene for normalization. Date is expressed as mean ±S.D from at least three independent determinations. * P<0.05, as compared with control; # P<0.01, as compared with control.