CIN is a consequence of RABL6A loss in fibroblasts.

<p>p53−/− MEFs expressing CON or RABL6A shRNAs (kd1 and kd2) were infected with vector (V) or human RABL6A (R, black bars) rescue viruses. (A) Representative metaphases of CON, kd1 and kd2 cells expressing empty vector. (B) Metaphases (n = 40) were counted and the percent of cells with <u>></u>80 chromosomes graphed. Data were analyzed using a Fisher’s exact test (*, p<0.0085). (C) The frequency of micronuclei formation under each indicated condition was quantified from three or more experiments with error bars representing the standard deviation from the mean. Statistical significance was determined using two-way ANOVA analysis (*, p<0.01 compared to CON; **, p<0.05 for (R) versus (V) cells).