Distribution of ON bipolar cell marker (PKCα) in the retina after serum injection.

<p>Wild type mouse retina 24 hours after control serum injection (A) and wild type mouse retina 5 hours (B) and 24 hours (C) after intravitreal injection with the patient’s serum. Retina from TRPM1 knockout mouse 24 hours after intravitreal injection with the patient’s serum.(D) Mouse retinas were stained with anti PKCα antibody (green) and co-stained with DAPI (blue) in the high magnification micrographs (A-C, right). Photomicrographs of the regions outlined by the white boxes are enlarged either to the right or the below the original images. The peripheral retina is oriented to the left and the central retina to the right. PKCα staining can be seen in the entire retina at both 5 hours after the patient serum injection and 24 hours after control serum injection (A and B, arrows). But the PKCα staining is mainly absent 24 hours after the injection of the patient’s serum (C, asterisk) and remained in only the peripheral retina (C, arrows). PKCα staining can be seen 24 hours after the injection of the patient’s serum in TRPM1 knockout mouse retina (D arrows). High magnification micrograph showed that the PKC α positive cell bodies were located mainly on the photoreceptor side of the INL. The scale bars are: 60 µm for A left, B left, C upper left and D left; 20 µm for A, B, C and D right; 30 μm for C lower left.