Selective sciatic motor neuron labeling for assessment of relative axonal misdirection.

<p>For demonstration purposes, representative coronal cut hemi-cord examples (stacks of all spinal cord sections of a single animal from each group) show the disorganization of labeled neurons after more severe nerve injuries, compared to <i>Sham</i>_{<b><i>s</i></b>} (stack of all 6 sham cords used to define the reference boundaries). FB (blue) or Di-I (yellow) labeled cells outside the reference bounds have axons misdirected respectively to the MG or sural nerves. Caudal sciatic pool boundaries were aligned to overlay the <i>Sham</i>_{<b><i>s</i></b>} group pool reference grid for the determination of motor neurons with misdirected axons (500µm scale bar).