Experimental procedure of antibody suspension bead arrays.

<p>The process starts with the distribution of samples into microtiter plates according to a defined, randomized sample positioning (A). The protein content of diluted samples is then labeled with biotin (B) and antibodies are coupled onto beads with distinct color codes to create a suspension bead array (C). Beads and samples are combined for incubation after the samples have been heat treated in assay buffer (D). Proteins that have not been captured by antibodies are removed and fluorescent streptavidin is added for detection (D). The beads are then measured and the co-occurrence of beads, which are identified via a green laser, and the emitted reporter fluorescence, excited by a red laser, allow the determination of interaction dependent intensity values in multiplex (E).