Differential removal of dying UV cones in zone 1 and 2.

<p><b>A</b>: Overview image of a retinal section from an opn1sw1:GFP transgenic animal at 1 and 2 days post diffuse light lesion (<b>A′</b>). <b>B</b>: Unlesioned red/green double cones are labelled by zpr-1 (red) and UV cones by GFP. Nuclei were stained with DAPI (blue). <b>B′:</b> Close up of outer segments (OS), nucleus (N) and pedicle (P) is in the OPL. <b>C:</b> Central retina (zone 1) showing removal of zpr1+ cones while disrupted UV cones persist (arrow). <b>C′:</b> DIC image showing the distribution of pigmented granula in the RPE (yellow bar) relative to Bruch's membrane (red dashed line). <b>C″:</b> Inset shows pyknotic nuclei in DAPI channel. <b>D:</b> Peripheral lesion with intact red/green cones but depleted UV cones. <b>D′:</b> DIC image showing condensed RPE pigments. <b>D″:</b> Co-localisation of pigmented processes with remaining GFP debris. ONL: Outer Nuclear Layer; RPE: Retina Pigmented Epithelium. Scale bars represent 500 µm.