Identification of extracellular proteins from <i>Clostridium difficile</i> culture supernatants.

<p>(<b>A</b>) Family distribution of putative surface/released proteins detected in 630 and R20291 culture supernatants. Proteins identified have been divided in families based primarily on the presence of motifs for association to the peptidoglycan and secondly on the presence of conserved functional domains. (<b>B</b>) Proteins detected by LC-MS/MS in <i>C. difficile</i> 630 and R20291 culture supernatants. Presence of a signal peptide motif was predicted with SignalP 4.0 using the 630 amino acid sequence (except for CDR20291_2278, for which a sequence with amino acid identity >40% is not present in 630). Cellular localization was predicted by PSORTb 3.0 using the 630 amino acid sequence (except for CDR20291_2278). Detection in 630 or R20291 culture supernatants is indicated by ‘yes/no’.