Representative 2D ¹H-¹³C HSQC HRMAS NMR spectra (A), zoomed in on the ATP region (B), obtained from a sample of a VHL-related abdominal PGL.

<p>The 2D NMR experiment confirms the presence of ATP in tumoral tissue as suggested by the 1D spectra of the same patient (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080539#pone-0080539-g001" target="_blank">Figure 1C</a>). Glutamate and norepinephrine assignments are also indicated. Because of their low concentration in the tissular sample, both succinate and glutathione are not clearly visible in the whole 2D spectrum (7.7-0.7 ppm in ¹H dimension) without focusing on their characteristic regions.