cGMP production by mouse TG cultures.

<p>A, ELISA-based quantification of intracellular cGMP in primary cultures from P12 mice. Cultures were treated with BNP (100 or 500 ng/ml for 5, 10 or 30 min) and/or anantin (100 and 500 nM, 30 min), or treated with vehicle (indicated as 0). Note large rise in cGMP that declines at 60 min; anantin alone (500 nM) decreases basal cGMP. Data were normalized to the total protein content in each sample and are presented as mean value ± SEM (n=3). *p≤0.05. B (top), example of western blot of Akt phosphorylation induced by BNP (500 ng/ml) applied alone or together with anantin (100 and 500 nM) for various times as indicated. Bottom histograms quantify these data from at least 3 experiments, demonstrating early and transient increment in P-Akt expression.