J100D expression of mIL-15 and mIL-15Rα increases mIL-15 production and results in soluble mIL-15/IL-15Rα complex.

<p>A) Western blot demonstrating mIL-15 production in Neuro-2a cells infected by J100 or J100D at a MOI of 10. B) ELISA demonstrating the presence of mIL-15/IL-15Rα complex in the supernatant of Neuro-2a cells infected by J100D, but not J100, at an MOI of 10. C) Quantification of mIL-15/IL-15Rα concentration in the supernates of J100D infected GL261 or CT-2A cells at the indicated hpi and MOIs. No mIL-15/IL-15Rα was detected in the supernates of J100 infected GL261 or CT-2A cells at any timepoint or MOI (not shown). The concentration of mIL-15/IL-15Rα in the supernates of CT-2A cells infected by J100D is significantly higher than the concentration in the supernates of infected GL261 cells at all MOIs and timepoints analyzed (<i>p</i> < 0.05).