BMX inhibited serum-induced cell proliferation in HCT116 cells.

<p>(A) HCT116 cells were treated with vehicle or BMX at indicated concentrations for 24 or 28 h. Cell viability was then determined by MTT assay. Each column represents the mean ± S.E.M. of seven independent experiments performed in duplicate. (^*p<0.05 as compared with the vehicle-treated control group) (B) After starvation in serum free medium for 24 h, HCT116 cells were treated with indicated concentrations of BMX in the presence of serum (10% FBS) for another 24 h. Cell viability was then determined by MTT assay. Each column represents the mean ± S.E.M. of five independent experiments performed in duplicate. (^*p<0.05 as compared with the vehicle-treated control group in the presence of serum) Cells were treated for 24 h with vehicle or BMX at indicated concentrations. Protein levels of p21 (C) and survivin (D) were then determined by immunoblotting. Compiled results are shown at the bottom of the chart. Each column represents the mean ± S.E.M. of five independent experiments. (^*p<0.05 as compared with the vehicle-treated control group).