Schematic genetic maps of pDSK5191 and its derivative expression plasmids.

<p>(A) Genetic map of the IncQ-group conjugation plasmid pDSK5191. Protein coding sequences are shown as block arrows. The pale gray rectangle represents the region of Ω-cassette in which T4-phage transcription and translation terminator sequences [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082345#B23" target="_blank">23</a>] are located at both ends. “Ori” represents the region containing the <i>oriV</i> and <i>oriT</i> sequences, which are derived from RSF1010 and are required for replication and mobilization of the plasmid, respectively [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082345#B15" target="_blank">15</a>]. (B) Expression constructs of the pDSK5191-derivative plasmids. Each construct was inserted into the unique <i>Eco</i>RI site of pDSK5191, as indicated by the arch-shaped arrow at the top of the panels. Protein-coding sequences are shown as block arrows. The pale gray rectangle represents the six-consecutive histidine-tag (6xhis) attached to the 5’ end of the <i>pscA</i> gene. “P_<i>pscA</i>” and “ter” are a putative constitutive promoter and a ρ-independent transcription terminator, respectively, of the <i>C. tepidum </i><i>pscAB</i> gene cluster.