Representative specular microscopy images of endothelial cells before and after cataract surgery.

<p>(<b>A</b>) Prior to surgery, the endothelial cells were relatively uniform in size and shape. (<b>B</b>) Due to corneal edema on Day 1 after surgery, the posterior surface of cornea was uneven, and it was difficult to capture clear images for all of the endothelial cells. The entire endothelial layer was not at the same focus plane, which indicates DEC swelling. A similar finding was also evident in UHR-OCT images (refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080986#pone-0080986-g001" target="_blank">Figure 1C</a>). Nevertheless, the loss of endothelial cells and the increased size of the cells were evident when compared to the pre-operative endothelium. (<b>C</b>) The larger cells and decrease in cell density were still evident on Day 7 after surgery. Bars = 50 μm.