Cocaine-induced phosphorylation of striatal DARPP-32 at Thr-34 and Thr-75 are oppositely affected in striatum-A_2AR KO and forebrain-A_2AR KO mice.

<p>Western blot analysis of phosphorylated (p-Thr-34 and p-Thr-75) and total DARPP-32. Representative Western blots of striatal protein extracts from fb-A_2AR KO and fb-WT (<b>A</b> and <b>E</b>), st-A_2AR KO or st-WT mice (<b>C</b> and <b>F</b>). The levels of DARPP-32 phosphorylation (normalized with total DARPP-32 level) are shown as mean ± SEM and presented as percentage of the value for saline-treated WT mice, for p-Thr-34 DARPP-32 levels in <i>fb</i>-A_2AR KO (n = 4, <b>B</b>) and <i>st-</i>A_2AR KO (n = 6, <b>D</b>) and p-Thr-75 DARPP-32 levels in <i>fb</i>-A_2AR KO (n = 4, <b>F</b>) and <i>st-</i>A_2AR KO (n = 6, <b>H</b>). # p<0.05 comparing cocaine with saline treatment within same genotype, two-way ANOVA and a <i>post hoc</i> Bonferroni test; * p<0.05 comparing fb-A_2AR KO or st-A_2AR KO with their corresponding WT littermates with same treatment, two-way ANOVA <i>post hoc</i> Bonferroni test.