mRNA levels of AgRP, CART, NPY, ObRb, POMC and SOCS-3 in hypothalamus in the offspring.

<p>Hypothalamic expression levels of AgRP, CART, NPY, ObRb, POMC and SOCS-3 in 25-day-old male and female offspring of dams with free access to standard chow diet (controls), the offspring of 20% calorie restricted dams during the first 12 days of pregnancy (CR), and CR rats daily supplemented with physiological doses of leptin throughout lactation (CR-Leptin). mRNA levels were measured by qRT-PCR and expressed as a percentage of the value of control male rats. Data are mean ± S.E.M. (n = 10-11, coming from at least six different litters). Statistics: *, CR different from control group (p < 0.05; Student’s <i>t</i>-test); in case of interaction within each sex, bars not sharing a common letter (a and b) are significantly different (a≠b) (p < 0.05; LSD <i>post </i><i>hoc</i> one-way ANOVA test).