The effect of treatment on serum anti-dsDNA IgG and renal immune complexes.

<p>(A), Anti-dsDNA IgG levels for mice in saline (n=13), Dex (n=13), and P-Dex (n=9) treatment groups were determined via ELISA at pretreatment, 4-week, 8-week, and 12 week time points. For the saline group, serum was available for analysis only from the subset of mice surviving at each time point: 12 mice at 4-week time point; 11 mice at 8-week time point; 9 mice at 12-week time point (B), Levels of anti-dsDNA IgG of each subclass were determined via ELISA at the 12-week time point. For the saline group, serum was available for this analysis only from the 9 mice that survived to the 12-week time point (C), Representative sections of kidney from each treatment group are shown. Sections were stained for renal deposition of anti-dsDNA IgG via immunohistochemistry. (D), Quantification of immune complex staining is illustrated. Scale bars: 25 μm; The asterisk (*) indicates a statistically significant difference (<i>P</i> < 0.05) from the saline control group. The dagger (†) indicates a statistically significant difference (<i>P</i> < 0.05) from the pretreatment time point of the same treatment group.