The lack of AGS3 does not affect autophagy induction in primary mouse macrophages.

<p>(<i>A</i>) Immunoblot analysis of cell lysates from BMDM prepared from wild type mice or Gpsm1^-/- mice to assess the steady state levels of Gα_i3, Gα_i2, p62 and ubiquitin proteins. (<i>B</i>) Immunoblot analysis of cell lysates from BMDM prepared from wild type mice or Gpsm1^-/- mice either not manipulated, starved (HBSS for 1h with 100 nM Bafilomycin A1), or treated with nigericin (4 µM for 4h) to assess LC3-II/actin band ratios. (<i>C</i>) Endogenous LC3 immunocytochemistry of BMDM from wild type mice or Gpsm1^-/- mice either left untreated, starved (HBSS for 1h with 100 nM Bafilomycin A1), or treated with nigericin (4 µM for 4 h). Representative images are shown for each condition. (<i>D</i>) Quantification of endogenous LC3 dots performed by fluorescence microscopy for at least 70-100 cells from experiment shown in part C. Data represents the mean LC3 puncta per cytosol ± SEM for three independent experiments for each condition.