Embryonic cultured cortical neurons express Cend1, RanBPM and Dyrk1B.

<p>(<i>a</i>) Immunofluorescence labeling and confocal analysis of cortical neurons from E16.5 mouse embryos cultured for 8 days <i>in </i><i>vitro</i>. Cultures were double-labeled for the neuronal marker betaIII-tubulin (Tuj1 antibody; red) and Cend1, RanBPM or Dyrk1B (green), respectively, as indicated. The panels on the right show at higher magnification the areas marked by white rectangles in the merged figures. Scale bar: 20 μm. (<i>b</i>) Immunoblot analysis of cortical neuron lysates cultured for 8 days <i>in </i><i>vitro</i>, confirming the expression of Cend1, RanBPM and Dyrk1B.