γ-H2AX foci kinetics following split multiple radiation exposures.

<p>Number of γ-H2AX foci per cell after exposure to 1 Gy at time 0 plus 1 Gy delivered20 min later (panel A), 1 hour later (panel B), 2 hours later (panel C), 5 hours later (panel D) and 12 hours later (panel E) using 225 kV_p X-rays. In Panel F representative pictures of cells in different exposures scenario are presented, i.e. cells fixed after 30 minutes in the cases of 20 minutes split dose exposure (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079541#pone-0079541-g001" target="_blank">Figure 1</a>), 1 hour split dose exposure (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079541#pone-0079541-g002" target="_blank">Figure 2</a>), 2 hours split dose exposure (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079541#pone-0079541-g003" target="_blank">Figure 3</a>) and 5 hours split dose exposure (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079541#pone-0079541-g004" target="_blank">Figure 4</a>). Error bars represent one standard error of the mean of 3 independent experiments. Lines are guides for the eyes.