Lrp1 disruption in cardiovascular tissues and isolated cardiac myocyte function.

<p><b>A</b>, Immunoblots of Lrp1 in tissue and cell extracts from heart, aorta, liver and cardiomyocytes demonstrated disruption of Lrp1 protein in cardiovascular tissues of <i>sm22-cre</i> Lrp1^{<i>flox/flox</i>} mice. Cardiomyoctye mechanics were demonstrated in (<b>B</b>) representative cell shortening tracings of smLrp1^+/+ and smLrp1^-/- cardiomycytes, as well as (<b>C</b>) fractional shortening, (<b>D</b>) rates of relaxation, -d<i>L</i>/d<i>t</i>, and (<b>E</b>) rates of contraction, +d<i>L</i>/d<i>t</i>. n = 3 mice per group for Lrp1 immunoblots and n = 27 cells from 3 smLrp1^-/- hearts; n = 20 cells from 2 smLrp1^+/+ hearts. Data are mean ± SEM.