Effect of FABP4 on target genes regulated by c-fos and/or c-myc.

<p>(A) Effect of FABP4 on cyclin D1 expression. Proliferating HCASMC were incubated with 120 ng/ml FABP4 in the presence or absence of c-myc (10074G5) and c-jun (SP600125) inhibitors for 6 h. Western blotting was performed to evaluate the expression of cyclin D1 in the cells, with actin as an internal control. (B) Migrating HCASMC were incubated with 120 ng/ml FABP4 in the presence or absence of c-myc (10074G5) and c-jun (SP600125) inhibitors for 24 h. Total RNA was extracted from the cells, and the expression of fibulin 4, fibulin 5, MMP2, and CCL2 was measured by quantitative RT-PCR using TaqMan primers and the 2ΔΔCt method. *p<0.05 vs unstimulated cells; #p<0.05 vs FABP4-stimulated cells.