TLR5 Signaling is not Enhanced by SP-A Interactions with Flagellin.

<p>Immunoblot was used to compare the phosphorylated and non-phosphorylated TLR-5 signaling pathway including IκBα, ERK and p38 in cell lysates from MH-s cells following stimulation with SP-A, flagellin or flagellin with SP-A. Blot in panel A is a representative experiment. Densitometry was performed on three experiments with multiple replicates and expressed as the phosphorylated divided by the non-phosphorylated form (B, C, D). There were no significant differences between any of the stimulations. Data shown are from 20 minutes of stimulation, but there were also no differences between groups noted following 10, or 60 minutes of stimulation as well (data not shown).