CpG methylation in Region 2 of the <i>SLC6a2</i> promoter in humans.

<p>The methylation status of the <i>SLC6a2</i> promoter Region 2 (-180 bp to +167 bp) was analysed by bisulphite sequencing in 4 healthy subjects compared to 4 subjects with MDD and panic disorder. <b>A</b>) A schematic representation of Region 2. The individual CpG sites analysed in Region 2 are indicated as enlarged boxes with their positioning relative to the transcription start site (+1) indicated with an arrow. <b>B</b>) Bisulphite sequencing data are presented as in Figure 1. For healthy subjects #1-4, 10 individual clones were sequenced. For MDD subjects #1-4, between 8 and 9 individual clones were sequenced. A different MDD patient (#5) replaced sample #3 due to insufficient sample. For panic disorder subjects #1-4, between 8 and 10 individual clones were sequenced.