<i>H. pylori</i> survival in WT and Jα18^−/− mice and gene expression analysis of chemokines and transcription markers.

<p>The stomachs of WT (n = 4) or Jα18^−/− (KO) (n = 4) mice infected with <i>H. pylori</i> expressing αCgT variants were assayed for bacterial number and host response 10 days later. (A) Bacterial recovery from the stomach of <i>H. pylori</i>-infected WT or Jα18^−/− mice. (B) Expression of Vα14-Jα18 transcripts in stomach tissue specimens from WT mice as measured by RT-PCR. Tissue specimens are the same as those shown in panel A. (C, D, E, F) Expression of IFN-γ (C), IL-12p40 (D), IL-4 (E), and Foxp3 (F) as assayed by RT-PCR. The same cDNA samples used in panel B were used in C through F. Transcript levels were normalized to the β-actin signal. PC in (F) stands for positive control for Foxp3. Statistical analysis was performed using Prism 5 and an unpaired <i>t</i>-test (*, <i>P</i><0.05). Means ± S. D. are shown.