<i>Giardia</i> trophozoites in the 21-day co-culture.

<p>The co-culture model was assembled as described. <b>A</b>) Live images of the model with parasites attached were obtained at 1 and 21 days. A trophozoite is identified with an arrow. Full size images of the model at day 1, 5, 13, and 21 are provided in the supplemental data (Figures S3–S6). <b>B</b>) <i>Giardia</i> growth curve over 21 days in the co-culture model. Trophozoites removed from the inserts with formononetin treatment were collected and counted with a hemocytometer at 1, 5, 13, and 21 days. Data represents the mean of four individual insert counts ± SD.