Effect of <i>Giardia</i> on Caco-2 cell number.

<p><i>Giardia</i> trophozoites were added to the co-culture at 100,000 total parasites for 5 days in 90% DMEM/10% <i>Giardia</i> media. Methylene blue staining of Caco-2 cells alone, Caco-2 cells (+) macrophages, Caco-2 cells (+) <i>Giardia</i>, Caco-2 cells (+) macrophages (+) <i>Giardia</i>. Values are expressed as the% control of Caco-2 cells alone ± SEM. (p<0.0001) (n = 3).