HT-1080s and WM239a cells are characterized by rear-end MCAM-expressing uropod like structures.

<p>Illustration of uropod-like features and MCAM expression for tumor cells in synthetic ECM (220 Pa, 1000 μM CRGDS): (<b>A</b>-<b>C</b>) Z-projected immunofluorescence images for a WM239a melanoma cell; (<b>A</b>) β1-integrin (green), counterstained with TRITC-conjugated phalloidin (F-actin, red) and DAPI (nucleus, blue); Single channel images (grayscale) illustrate (<b>B</b>) Factin and (<b>C</b>) β1-integrin. (<b>D</b>) Time-lapse images for a GFP-MCAM transfected WM239a melanoma cell (30 min / frame; See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081689#pone.0081689.s024" target="_blank">Movie S13</a>); Brightfield (top) and GFP-MCAM expression (bottom). (<b>E</b>-<b>G</b>) Z-projected immunofluorescence images for a WM239a melanoma cell; (<b>E</b>) Melanoma cell adhesion molecule (MCAM, green), counterstained with TRITC-conjugated phalloidin (F-actin, red) and DAPI (nucleus, blue); Single channel images (grayscale) illustrate (<b>F</b>) Factin and (<b>G</b>) MCAM. (<b>H</b>) Time-lapse images for a GFP-MCAM transfected HT-1080 cell (15 min / frame; Background pixel intensity subtracted using the “Math” function in ImageJ and uniformly applied to all GFP images for better display); Brightfield (top) and GFP-MCAM expression (bottom).