Effect of rapamycin on autophagy and autophagic flux in normal mice.

<p>Using an antibody that identified both LC3B-I (top band) and -II (bottom band), representative Western blots and grouped data are presented for myocardial LC3B-II levels in normal mice treated with placebo (n = 4), 8 mg/kg/day PO rapamycin (n = 5) or rapamycin plus chloroquine (10 mg/kg IP, n = 4) to assess autophagic flux. Data are mean ± SEM. †: <i>P</i><0.05 vs placebo, ‡: <i>P</i><0.05 vs. rapamycin.