Characterization of the transgenic rice seedlings under cold treatment.

<div><p>A. 0 day - germinated seeds of WT and transgenic plants; CK - seedlings of the WT and transgenic plants grown for 6 d at room temperature (28°C); Cold - seedlings with 5 mm bud burst were treated at 4-5°C for 7 d, then recovered for 4 d at room temperature (28°C); Cold+GSH - the cold-treated seedlings were recovered under 10 mg/L GSH solution for 4 d at room temperature (28°C). A1 and A2 are Ubi<i>::OsSPX1</i>-antisense transgenic lines (on the left), S1 and S2 are Ubi<i>::OsSPX1</i>-sense lines (on the right).</p> <p>B. The height of the WT and transgenic plants over the recovery time after cold treatment (blue lines for Ubi<i>::OsSPX1</i>-antisense and red lines for Ubi<i>::OsSPX1</i>-sense).</p> <p>C. The survival rate of the WT and transgenic plants after cold treatment, with/without GSH.</p> <p>D. H₂O₂ content of the leaves of WT and transgenic rice plants under cold treatment (blue bars for Ubi<i>::OsSPX1</i>-antisense and red bars for Ubi<i>::OsSPX1</i>-sense).</p> <p>E. Peroxidase activity in the WT and transgenic rice plants under cold treatment (blue bars for Ubi<i>::OsSPX1</i>-antisense and red bars for Ubi<i>::OsSPX1</i>-sense).</p> <p>** represent significant differences (p < 0.01) between transgenic and WT rice plants according to Student’s <i>t</i>-test.</p>