Competitive binding assays of the Cry8 toxin and toxin-DNA complex using the BBMVs from the target larvae.

<p>A. Competitive binding of the Cry8Ea1 toxin and Cry8Ea1 toxin-DNA complex with the BBMVs of <i>H. parallela</i> larvae. B. Competitive binding of the Cry8Ca2 toxin and Cry8Ca2 toxin-DNA complex with the BBMVs of <i>A. corpulenta</i> larvae. Lane 1, homologous competitive binding of the Cry toxin and biotinylated Cry toxin; Lane 2, homologous competitive binding of the Cry toxin-DNA complex and biotinylated Cry toxin-DNA complex; Lane 3, heterologous competitive binding of the Cry toxin-DNA complex and biotinylated Cry toxin; and Lane 4, homologous competitive binding of the Cry toxin and its biotinylated Cry toxin plus calf thymus DNA (0.2 µg in each 100-µl aliquot of competitive binding reaction) as an external DNA control.