Decreased proliferation and change in physical properties of mutant optic vesicle.

<p>(A) Immunohistochemical analysis for the presence of BrdU-labelled cells in the prospective neural retina on a coronal section of an E10.5 control embryo (left panel) and an <i>Lhx2-Cre:β-catenin^flox/flox</i> embryo (right panel). The part of the prospective neural retina that was analysed for BrdU⁺ cells in the control and mutant embryos are outlined. (B) Immunohistochemical analysis for the presence apoptotic cells as revealed by the presence of activated Casp-3⁺ cells on coronal section of an E10.5 control embryo (left panel) and a <i>Lhx2-Cre:β-catenin^flox/flox</i> embryo (right panel). (C) Immunohistochemical analyses for the presence of phosphorylated myosin light chain 2 (pMLC2) on a coronal section of an E10.5 control embryo. (D) Immunohistochemical analyses for the presence of pMLC2 on a coronal section on an E10.5 <i>Lhx2-Cre:β-catenin^flox/flox</i> embryo. Insets in C and D are a magnification of the areas indicated by the squares. Scale bars: (A, B and C, D) 100 µm.