Capsule production and HD-5 resistance in EPEC and EHEC.

<p>(A) Transcription of <i>gfcA</i> in the EPEC and EHEC wild-type strains was quantified by qPCR. Data shown (2^-ΔCT x 10³) are representative of <i>gfcA</i> gene expression normalized against 16S RNA gene expression. Results are expressed as means ± SEs of triplicate samples. Asterisks indicate statistical significance; **, <i>P</i> <0.01 by paired <i>t</i> test. (B) Capsule staining of EPEC and EHEC wild-type strains, capsules are visualized by negative staining at a magnification of 100 X. Images shown are representative of at least ten fields of view from three independent experiments. (C) Survival of EPEC and EHEC wild-type cells in the presence of 5 µM HD-5. Results are expressed as means ± SEs of triplicate samples. Data shown are representative of at least three independent experiments. Asterisks indicate statistical significance; **, <i>P</i> <0.01 by two-way ANOVA and Bonferroni's multiple comparison <i>post </i><i>hoc</i> test.