IGF1R expression rescue from miR-223 suppression.

<div><p><b>A</b>&<b>B</b>. The mRNA expression of IGF1R (A) and Sp1 (B) was monitored by qRT-PCR examination in HeLa or HEK-293T cells transfected with pLL3.7-miR-223, psiCHEK-2-IGF1R3’UTR and decoy nucleotides. The expression levels were normalized to GAPDH which served as the loading control. </p> <p><b>C</b>&<b>D</b>. The mRNA expression of IGF1R (C) and Sp1 (D) was measured in SMMC-7721 cells transfected with pLL3.7-miR-223, psiCHEK-2-IGF1R3’UTR and decoy nucleotides.</p> <p><b>E</b>&<b>F</b>. The protein levels of IGF1R (C) and Sp1 (D) in SMMC-7721 cells co-transfected with miR-223 and decoy nucleotides (upper), and quantified by densitometry (lower). GAPDH served as an interior loading control. The data are representative from 3 independent experiments. **, <i>P</i><0.01 .</p>