Colonization of uropathogenic <i>E. coli</i> during systemic disseminated infection in mice.

<p>(A) Bacterial load in the spleens and livers of mice at 24 hpi. CBA/J mice were inoculated with indicated doses of the wild-type <i>E. coli</i> strain CFT073 via the tail vein. Each data point indicates CFU count obtained from an individual mouse. (B) Twenty mice were inoculated with Tn<i>5</i> transposon mutant pool and the bacterial load in spleens is depicted. These colonies were harvested for genomic DNA extraction and used for determination of transposon insertion sites by targeted sequencing.