Cell delivery of ssDNA mediated by DENV C protein-derived peptides (pepR and pepM).

<p><b>A)</b> Confocal imaging of BHK-21 cells. Cells were cultured for two days and then stained with Hoechst 33342 at 1 mg.mL⁻¹ (blue – nuclei), followed by the addition of 5 µM of either pepR-rhodamineB or pepM-rhodamineB (red) conjugated with 1 µM of ssDNA-Alexa488. <b>B)</b> Flow cytometry quantification of the cellular internalization of ssDNA labeled with Alexa488, by using pepM, pepR, DENV C protein or Tat as delivery vectors, in BHK-21 cells.