Seropositive samples by ELISA to three different strains of CNV.

<p>Canine serum samples that were seropositive to pooled CNV VLPs were screened against individual CNV VLP strains; 170, C33 and HK. (A) Venn diagrams represent seroprevalence of each CNV strain in the 1999–2001 and the 2012–2013 cohorts. The number of dogs seropositive to one strain alone or combinations of strains are represented as percentages. (B) The relative OD450 value to each CNV strains of each dog are represented by heatmaps. Each column represents a single dog. Positive threshold value was established from the mean OD450 of coating buffer alone plus three standard deviations. Relative increase in OD450 values above the positive threshold were calculated to enable fair comparison between experiments. A relative increase of <1 indicates a seronegative sample, represented by a white box. The degree of relative increase for samples is represented by increasing darkness of the corresponding box.