Increase of cell invasion following FILIP1L knockdown in various FILIP1L-high-expressing, low-invasive cancer cell lines.

<p>MCF7 breast (<i>B</i>), NCM460 colon (<i>C</i>), H661 lung (<i>D</i>) and Capan-1 pancreatic (<i>E</i>) cancer cell lines were transfected with either non-targeting or <i>FILIP1L</i> siRNA. <i>A</i>, Immunoblot analysis for FILIP1L in the transfected cells. GAPDH blot is shown as the loading control. <i>B</i>-<i>E</i>, Transfected cells were subject to Matrigel cell invasion assay 48 h after transfection. The same experimental procedures were followed as described in Figure 6. The result is representative of two independent experiments.