Surrogate anti-CCL17 antibodies neutralize mouse CCL17 calcium mobilization in a dose-dependent manner.

<p>CCRF-CEM cells were treated in quadruplicate with mouse CCL17 at 2 ng/mL in the presence of either B202 (<i>solid line/squares</i>) or B225 (<i>dotted line/circles</i>) with each antibody at a starting concentration of 10 µg/mL. The IC₅₀ of each antibody was calculated using GraphPad Prism non-linear regression analysis and determined to be as follows: B225 63.05 ng/mL; B202 225.4 ng/mL. Results are representative of 3 experiments.