Validation of the ligand-targeted PCR method for CTC detection.

<p>A) Recovery ratio of spiked cell assay. B) Linear regression curve of spiked cell assay. C) Specificity test of the assay. Spiked KB cells were treated with (<i>competed</i>) or without (<i>control</i>) 10 µM folic acid before labeling. D) Healthy donors' blood samples spiked with 20 KB cells <i>(20 cells)</i> or without KB tumor cells <i>(control)</i> were labeled by 10 nM folate-oligonucleotide conjugate <i>(conjugate)</i> or unconjugated-oligonucleotide <i>(oligonucleotide)</i> after enrichment. Data are shown with Mean±SD from three independent assays.