DNA topology during the IVR.

<p>(<b>A</b>) Various forms of the substrate plasmid were analysed on a 0.8% agarose gel at 5–10 V/cm for 16 h at 4°C. scDNA (sc) migrated faster than linear (L), while nicked open circular or relaxed circular DNA (oc/rc) migrated slowest. (<b>B</b>) Each topological form of DNA substrates was subjected to IVR without AID-induced damage. Experiments were done as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082097#pone-0082097-g002" target="_blank">Figure 2</a>, and results expressed as % of input. Error bars indicate ± SD (n = 3). (<b>C</b>) Modulation of DNA topology due to FE. 1 µg of supercoiled DNA (lane 1) was incubated with FE (lane 2) and analysed as in (A).