Gene expression measured by quantitative real time polymerase chain reaction (qRT-PCR) after 240 hrs of replenishing conditioned media treatment.

<p><b>A.</b> Significantly increased (*p<0.03) expression of collagen I, fibronectin, α-SMA, CTGF, PAI-1 and TGFβ mRNA was observed in NF treated with PKF or IKF conditioned media (versus NF control conditioned media) after 240 hrs. All mRNA expression was normalised to the internal reference gene RPL-32. <b>B.</b> Increased gene expression was observed for NS treated with PKF or IKF conditioned media versus NS control media after 240 hrs. NF = normal dermal fibroblasts (n = 4), NS = normal dermal scar fibroblasts (n = 4), PKF = peri-lesional keloid fibroblasts (n = 5), IKF = intra-lesional keloid fibroblasts (n = 5).