Lymphocytes with naive phenotype in cord blood and intestine.

<p>Flow cytometry of CBMCs from normal and FOXN1^−/− fetuses matched for gestational age (16 weeks of gestation). Dot plots show the expression pattern of the naïve cell markers. (A) Frequencies of CBMCs expressing both CD45RA and CD3 markers. (B) Frequencies of CBMCs expressing both CD62L and CD3 markers. (C) Frequencies of CBMCs coexpressing CD45RA and CD62L markers. (D) Frequencies of CBMCs coexpressing CD45RA and CD27 markers. Experiment in (A), (B), (C) and (D) was repeated two times. Data were obtained by gating first on viable cells and later on CD45⁺ cells (A and B) or finally also on CD3⁺ (C and D). (E) Confocal microscopy of fetal intestinal sections labeled with anti-human CD3 (red) and anti-human CD45RA (green). Representative results from three independent experiments with two samples are shown.