Between weeks 11 and 13, approximately 70% of the mice (<i>n</i>  =  28) implanted with MDSPCs formed large neoplastic growths.

<p>(<b>A, B</b>) Representative image of hematoxylin and eosin staining of tumors that formed in mice implanted with MDSPCs. (<b>C-L</b>) The resulting tumors were classified as malignant peripheral nerve sheath tumors with rhabdomyoblastic differentiation (Triton tumors) by showing positivity for (<b>C, D</b>) smooth muscle actin (brown), (<b>E, F</b>) desmin (brown), (<b>G, H</b>) NF (brown), and pockets of (I, J) S100 (brown), (<b>K, L</b>) as well as cells positive for both NF (brown) and S100 (purple). Positive cells (brown, <b>C-J</b>) or double-positive cells (K and L) are indicated by black arrows. Images B, D, F, H, J, and L show a higher magnification image of the image they were preceded by. (<b>M, N</b>) The neoplasias were also positive for FluoroMyelin (green), showing areas of unorganized myelin deposition. (<b>O</b>) Image depicting NF (green) and FluoroMyelin (red) double-stained sections from a regenerated nerve (white arrow) in the area of tumorigenesis. Negative control sections were similarly processed without primary antibodies. Scale bars represent 100 µm.