CNrasGEF enhances the inhibition effect of NEDD4-1 downregulation on cell migration and invasion.

<p>A) Western blotting showed the expression of NEDD4-1 and CNrasGEF after co-transfection in U251 cells. Representative western blotting analysis of total cell lysates isolated from U251 cells co-transfected with shNEDD4-1 and CNrasGEF using indicated antibodies. B) Quantitative analysis of relative protein levels of NEDD4-1 and CNrasGEF normalized to those of β-actin. C) Wound-healing assays of glioma U251 cells after co-transfection of shNEDD4-1 and CNrasGEF. Representative digital pictures were taken at 0h and 24h. D) Quantitative analysis of the number of migratory cells. Results are mean ± SEM of three independent experiments in triplicate. *<i>P</i><0.05, ** <i>P</i><0.01. Scale bar: 100μm.