Culture expanded CD45^- MHCII^lo/-EpCAM^lo Sca1⁺ CD49F^loCD29⁺H2BGFP LRCs maintain a surface phenotype similar to mesenchymal stem cells.

<p>After expanding sorted EpCAM^lo LRCs for 5 passages <i>in </i><i>vitro</i>, their cell surface profile for a panel of TEC and mesenchymal stem cell surface proteins using flow cytometry. Overlay histograms show a comparison of staining for each antibody including EpCAM, MHCII, H2BGFP, Sca1, CD49F CD29, CD44, CD34, PDGFRα PDGFRβ, CD90 and SSEA in open histograms with the relevant isotype control antibody conjugated to the same fluorochrome in gray shaded histograms. The negative control for H2BGFP expression was TMSCs derived from C57BL/6J mice.