<i>In vitro</i> growth potential of H2BGFP LRCs.

<p>Methylene blue stained colonies derived from FACS sorted CD45^- EpCAM^lo MHCII^lo/-Sca1⁺H2BGFP+ LRCs (A) or CD45^-MHCII^intEpCAM^hi Sca1⁺ H2BGFP⁺ stroma (B). C. Merge of H2BGFP expression and phase contrast image of expanded EpCAM^loMHCII^lo/- H2BGFP⁺ LRCs 1 week after sorting, demonstrating dramatic <i>in </i><i>vitro</i> growth. D. Merge of H2BGFP expression and phase contrast image showing limited expansion of EpCAM^hi MHCII^intH2BGFP⁺ LRCs 1 week after sorting. E. H2BGFP expression in same field as C. F. H2BGFP expression in same field as D. These results are representative of 3 independent experiments performed with 10-12 week Dox fed H2BGFP mice. Methylene blue stained colonies derived from FACS sorted CD45^-MHCII^lo/-EpCAM^lo Sca1⁺CD49F^lo CD29⁺ (G) or CD45^-MHCII^intEpCAM^hiSca1⁺CD49F^hiCD29⁺ stroma (H) derived from WT C57BL/6J mice. I. Colony forming potential of sorted LRCs and defined TEC subsets sorted from dissociated thymus derived from postnatal C57BL/6J mice. Error bars show standard deviation of means calculated from 5 independent experiments. P values are derived by comparison of colony forming potential of sets of populations using T test.