Validation of selected genes by qRT-PCR.

<p>Gene IDs refer to the corresponding cDNAs from Genbank. qRT-PCR was performed on each sample in triplicate. <i>β-actin</i> was used an endogenous control. Ratio-RPKM used for comparing the difference of gene expression among samples and validation by qRT-PCR to show a certain consistent between the two methods except several inconsistencies. It may be attributed to the experimental errors which caused by the biological replicas deficiency. Only one pooled RNA sequenced for each sample due to the limitations of specimen collection that might cause the experimental errors unpredictably. We will sequence more samples to eliminate the errors in the further research.