The Agl-Nfs machinery is sporulation-specific.

<p>(A) <i>nfs</i> and <i>glt</i> genes are differentially regulated during sporulation. Expression of NfsD, GltD, and AglQ as inferred from the measurements of relative single cell mCherry/sfGFP fluorescence intensities over time after sporulation induction. Shown are the average fluorescence intensity ratios (measured intensity/maximum intensity) of 25 cells for each time points. (B) Time course of AglQ-sfGFP (left), GltD-mCherry (middle panel), and NfsD-mCherry (right panel) dynamics during sporulation.