BET-1 can be sumoylated <i>in</i><i>vitro</i>.

<p>A) A schematic map depicting BET-1 full length and the different fragments used in yeast two-hybrid and for the <i>in </i><i>vitro</i> sumoylation assay. B) The yeast two-hybrid assay shows that BET-1 and SMO-1 interact. The interaction requires lysine 252 within the C-terminal part of the bromodomain. The fragment from amino acid 614 to 853 auto activates and could not be tested for interactions. The photographs show the results from six serial (1 in 5) dilutions to allow semi-quantitative comparisons between conditions. Omitted amino acids are indicated on top left hand side. C) The <i>in </i><i>vitro</i> sumoylation assay analysed by Western blot against MBP shows that lysine 252 is sumoylated. The expected size of MBP fused to the bromodomain is roughly 80kDa and its sumoylated form corresponding to an added SUMO-1, branching out of the bromodomain, produces a shift of ~20kDa. The bands detected below are likely to be degradation products and/or incomplete translation products.